cAMP-dependent allostery and dynamics in Epac: An NMR view

Rajeevan Selvaratnam, Madoka Akimoto, Bryan VanSchouwen, Giuseppe Melacini

研究成果: Review article査読

19 被引用数 (Scopus)


Epac (exchange protein directly activated by cAMP) is a critical cAMP receptor, which senses cAMP and couples the cAMP signal to the catalysis of guanine exchange in the Rap substrate. In the present paper, we review the NMR studies that we have undertaken on the CBD (cyclic-nucleotide-binding domain) of Epac1. Our NMR investigations have shown that cAMP controls distal autoinhibitory interactions through long-range modulations in dynamics. Such dynamically mediated allosteric effects contribute not only to the cAMP-dependent activation of Epac, but also to the selectivity of Epac for cAMP in contrast with cGMP. In addition, we have mapped the interaction networks that couple the cAMP-binding site to the sites involved in the autoinhibitory interactions, using a method based on the covariance analysis of NMR chemical shifts. We anticipate that this approach is generally applicable to dissect allosteric networks in signalling domains.

ジャーナルBiochemical Society Transactions
出版ステータスPublished - 2012 2月

ASJC Scopus subject areas

  • 生化学


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