TY - GEN
T1 - Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production
AU - Tanaka, Aoi
AU - Takamori, Sho
AU - Mimura, Hisatoshi
AU - Osaki, Toshihisa
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Funding Information:
This work was partly supported by JSPS KAKENHI Grant Number JP21H05013, and by the Program for Building Regional Innovation Ecosystem of MEXT, Japan.
Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - This paper reports a cartridge-type device to produce sensor cells with spontaneous solution exchange. Our group has developed a cell-based odorant sensor expressed olfactory receptors. These sensor cells presented excellent sensitivity and specificity, which would be useful for a wide range of sensor applications. However, production of such sensor cells requires multiple times of aseptic solution exchanges. In this work, we develop a cartridge-type cell culture device that utilizes capillary force for spontaneous solution exchange without pumps or tubes. When the cartridge is set to the exchange stage, the specified volume of solution is exchanged immediately. We experimentally examine the solution exchange, and demonstrate the response of a sensor cell to a chemical stimulus in the exchanged solution. We consider that the device would be useful to produce multiple types of sensor cells.
AB - This paper reports a cartridge-type device to produce sensor cells with spontaneous solution exchange. Our group has developed a cell-based odorant sensor expressed olfactory receptors. These sensor cells presented excellent sensitivity and specificity, which would be useful for a wide range of sensor applications. However, production of such sensor cells requires multiple times of aseptic solution exchanges. In this work, we develop a cartridge-type cell culture device that utilizes capillary force for spontaneous solution exchange without pumps or tubes. When the cartridge is set to the exchange stage, the specified volume of solution is exchanged immediately. We experimentally examine the solution exchange, and demonstrate the response of a sensor cell to a chemical stimulus in the exchanged solution. We consider that the device would be useful to produce multiple types of sensor cells.
KW - Biohybrid odorant sensor
KW - Capillary force
KW - Insect olfactory receptor
KW - Sensor cell
KW - Solution exchange
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U2 - 10.1109/MEMS51670.2022.9699570
DO - 10.1109/MEMS51670.2022.9699570
M3 - Conference contribution
AN - SCOPUS:85126394889
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 166
EP - 167
BT - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Y2 - 9 January 2022 through 13 January 2022
ER -