TY - JOUR
T1 - CD24 is expressed specifically in the nucleus pulposus of intervertebral discs
AU - Fujita, Nobuyuki
AU - Miyamoto, Takeshi
AU - Imai, Jun Ichi
AU - Hosogane, Naobumi
AU - Suzuki, Toru
AU - Yagi, Mitsuru
AU - Morita, Kozo
AU - Ninomiya, Ken
AU - Miyamoto, Kana
AU - Takaishi, Hironari
AU - Matsumoto, Morio
AU - Morioka, Hideo
AU - Yabe, Hiroo
AU - Chiba, Kazuhiro
AU - Watanabe, Shinya
AU - Toyama, Yoshiaki
AU - Suda, Toshio
PY - 2005/12/30
Y1 - 2005/12/30
N2 - Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.
AB - Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.
KW - CD24
KW - Intervertebral disc
KW - Nucleus pulposus
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U2 - 10.1016/j.bbrc.2005.10.166
DO - 10.1016/j.bbrc.2005.10.166
M3 - Article
C2 - 16288985
AN - SCOPUS:27844438525
SN - 0006-291X
VL - 338
SP - 1890
EP - 1896
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -