TY - JOUR
T1 - CD300 antigen like family member G
T2 - A novel Ig receptor like protein exclusively expressed on capillary endothelium
AU - Takatsu, Hiroyuki
AU - Hase, Koji
AU - Ohmae, Masumi
AU - Ohshima, Sayaka
AU - Hashimoto, Koji
AU - Taniura, Naoko
AU - Yamamoto, Akitsugu
AU - Ohno, Hiroshi
N1 - Funding Information:
We thank Drs. T. Murakami, A. Yoshino, and K. Kawano for critical reading of the manuscript. This study was supported in part by Grants-in-Aid for Young Scientists (B) (H.T.), Scientific Research (H.O.), Scientific Research in Priority Areas (H.O.), and Protein 3000 Project (H.O.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2006/9/15
Y1 - 2006/9/15
N2 - We report the characteristics of CD300LG, a member of the CD300 antigen like family. Its genomic structure is similar in both mouse and human, and at least four isoforms exist in both species. The amino acid sequence of the immunoglobulin (Ig) V like domain of CD300LG showed approximately 35% identity to those of the polymeric Ig receptor (pIgR) and Fcα/μR. Interestingly, mouse CD300LG proteins were uniquely expressed on capillary endothelium. Immunoelectron microscopy revealed that mouse CD300LG is localized on both apical and basolateral plasma membranes, as well as on intracellular vesicular structures, in the capillary endothelium. Transcytosis assays using polarized MDCK epithelial cells showed that CD300LG could be transcytosed bidirectionally. Furthermore, CD300LG exogenously expressed on HeLa cells could take up IgA2 and IgM, but not IgG. These results suggest that CD300LG might play an important role in molecular traffic across the capillary endothelium.
AB - We report the characteristics of CD300LG, a member of the CD300 antigen like family. Its genomic structure is similar in both mouse and human, and at least four isoforms exist in both species. The amino acid sequence of the immunoglobulin (Ig) V like domain of CD300LG showed approximately 35% identity to those of the polymeric Ig receptor (pIgR) and Fcα/μR. Interestingly, mouse CD300LG proteins were uniquely expressed on capillary endothelium. Immunoelectron microscopy revealed that mouse CD300LG is localized on both apical and basolateral plasma membranes, as well as on intracellular vesicular structures, in the capillary endothelium. Transcytosis assays using polarized MDCK epithelial cells showed that CD300LG could be transcytosed bidirectionally. Furthermore, CD300LG exogenously expressed on HeLa cells could take up IgA2 and IgM, but not IgG. These results suggest that CD300LG might play an important role in molecular traffic across the capillary endothelium.
KW - CD300L
KW - CMRF35
KW - Capillary endothelium
KW - IgA
KW - IgM
KW - Immunoglobulin super family
KW - Transcytosis
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U2 - 10.1016/j.bbrc.2006.07.047
DO - 10.1016/j.bbrc.2006.07.047
M3 - Article
C2 - 16876123
AN - SCOPUS:33746760966
SN - 0006-291X
VL - 348
SP - 183
EP - 191
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -