TY - CHAP
T1 - CHAPTER 7
T2 - CE-MS for Anionic and Cationic Metabolic Profiling: System Optimization and Applications
AU - Hirayama, Akiyoshi
AU - Soga, Tomoyoshi
N1 - Funding Information:
This work was supported by aMeD-CresT from the Japan agency for Medical research and Development, aMeD, the research on Development of new Drugs (gapFree) from aMeD, and the Jsps kakenhI grant number Jp15k21364, as well as research funds from the yamagata prefectural government and the City of Tsuruoka.
Publisher Copyright:
© The Royal Society of Chemistry 2018.
PY - 2018
Y1 - 2018
N2 - Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be an important technology for metabolomics studies. Most primary metabolite intermediates contain hydroxyl, amino, carboxyl and phosphate groups and, therefore, are charged, which makes them suitable for CE-MS analysis. The major advantages of CE-MS are its high resolution and the fact that almost any charged metabolite (both cationic and anionic) can be analyzed by only two analytical modes. CE-MS can be readily applied to various kinds of biological samples. This chapter highlights the general CE-MS conditions specifically developed for profiling cationic and anionic metabolites, and also focuses on several biological samples frequently used in medical and biochemical metabolomics, including blood (serum and plasma), urine, tissue, cultured cells and other related samples.
AB - Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be an important technology for metabolomics studies. Most primary metabolite intermediates contain hydroxyl, amino, carboxyl and phosphate groups and, therefore, are charged, which makes them suitable for CE-MS analysis. The major advantages of CE-MS are its high resolution and the fact that almost any charged metabolite (both cationic and anionic) can be analyzed by only two analytical modes. CE-MS can be readily applied to various kinds of biological samples. This chapter highlights the general CE-MS conditions specifically developed for profiling cationic and anionic metabolites, and also focuses on several biological samples frequently used in medical and biochemical metabolomics, including blood (serum and plasma), urine, tissue, cultured cells and other related samples.
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U2 - 10.1039/9781788012737-00134
DO - 10.1039/9781788012737-00134
M3 - Chapter
AN - SCOPUS:85051491420
SN - 9781788010863
SN - 9781788011044
T3 - New Developments in NMR
SP - 134
EP - 160
BT - New Developments in NMR
A2 - Ramautar, Rawi
PB - Royal Society of Chemistry
ER -