Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast

Masako Inuyama, Fumihiko Ushigome, Akiko Emoto, Noriko Koyabu, Shoji Satoh, Kiyomi Tsukimori, Hitoo Nakano, Hisakazu Ohtani, Yasufumi Sawada

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[14C]lactic acid into basal membrane vesicles was Na+ independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). L-[14C]lactic acid uptake exhibited saturation kinetics with a Km value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and α-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4′-diisothiocyanostilbene-2,2′-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+ dependent and Na+ independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.

本文言語English
ページ(範囲)C822-C830
ジャーナルAmerican Journal of Physiology - Cell Physiology
283
3 52-3
DOI
出版ステータスPublished - 2002 9月
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 細胞生物学

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