Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam; Yonehiro Kanemura; Jesmin Tajria; Hideki Mori; Satoshi Kobayashi; Tomoko Shofuda; Jun Miyake; Masayuki Hara; Mami Yamasaki; Hideyuki Okano

doi:10.1016/j.febslet.2005.05.019

Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

Mohammed Omedul Islam
, Yonehiro Kanemura
, Jesmin Tajria
, Hideki Mori
, Satoshi Kobayashi
, Tomoko Shofuda
, Jun Miyake
, Masayuki Hara
, Mami Yamasaki
, Hideyuki Okano

生理学

研究成果: Article › 査読

50 被引用数 (Scopus)

抄録

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

本文言語	English
ページ（範囲）	3473-3480
ページ数	8
ジャーナル	FEBS Letters
巻	579
号	17
DOI	https://doi.org/10.1016/j.febslet.2005.05.019
出版ステータス	Published - 2005 7月 27

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/j.febslet.2005.05.019

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title = "Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells",

abstract = "We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.",

keywords = "ABC transporter, ABCB1, Cell surface marker, Neural stem cell",

author = "Islam, \{Mohammed Omedul\} and Yonehiro Kanemura and Jesmin Tajria and Hideki Mori and Satoshi Kobayashi and Tomoko Shofuda and Jun Miyake and Masayuki Hara and Mami Yamasaki and Hideyuki Okano",

note = "Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

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T1 - Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

AU - Islam, Mohammed Omedul

AU - Kanemura, Yonehiro

AU - Tajria, Jesmin

AU - Mori, Hideki

AU - Kobayashi, Satoshi

AU - Shofuda, Tomoko

AU - Miyake, Jun

AU - Hara, Masayuki

AU - Yamasaki, Mami

AU - Okano, Hideyuki

N1 - Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2005/7/27

Y1 - 2005/7/27

N2 - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

AB - We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.

KW - ABC transporter

KW - ABCB1

KW - Cell surface marker

KW - Neural stem cell

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AN - SCOPUS:21244452807

SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

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Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

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