TY - JOUR
T1 - Cis-acting elements involved in the G2/M-phase-specific transcription of the cyclin B gene in the unicellular alga Cyanidioschyzon merolae
AU - Sumiya, Nobuko
N1 - Funding Information:
I am grateful to Dr. Masaki Ito (Kanazawa University) for helpful advice during the study. This work was supported by JSPS KAKENHI Grant Number 17K18091, Basic Science Research Projects from The Sumitomo Foundation Grand Number 200251, and Keio University Academic Development Funds for individual Research, and the Research and Education Center for Natural Sciences, Keio University.
Funding Information:
I am grateful to Dr. Masaki Ito (Kanazawa University) for helpful advice during the study. This work was supported by JSPS KAKENHI Grant Number 17K18091, Basic Science Research Projects from The Sumitomo Foundation Grand Number 200251, and Keio University Academic Development Funds for individual Research, and the Research and Education Center for Natural Sciences, Keio University.
Publisher Copyright:
© 2021, The Botanical Society of Japan.
PY - 2021/11
Y1 - 2021/11
N2 - M-specific activator (MSA) cis-acting elements have been determined to be involved in the regulation of G2/M-phase-specific transcription in spermatophytes. In this study, the involvement of MSA-core elements in G2/M-phase-specific transcription was examined in the unicellular red alga Cyanidioschyzon merolae. In the C. merolae genome, MSA-core elements do not accumulate specifically in the upstream of mitosis-specific transcriptional genes. Mutations of the four MSA-core elements of the cyclin B gene, which encodes a central factor of the G2-to-M-phase transition, have resulted in the abolishment of transcription or permission of transcription even in the G1 phase. These results suggest that all four MSA-core elements located in the upstream region of cyclin B are involved in G2/M-phase-specific transcription in C. merolae; however, the nature of the involvement of MSA-core elements in G2/M-phase-specific transcription differed among the four elements. Thus, MSA-core-element-mediated G2/M-phase-specific transcription in C. merolae seems to be regulated by a complex mechanism.
AB - M-specific activator (MSA) cis-acting elements have been determined to be involved in the regulation of G2/M-phase-specific transcription in spermatophytes. In this study, the involvement of MSA-core elements in G2/M-phase-specific transcription was examined in the unicellular red alga Cyanidioschyzon merolae. In the C. merolae genome, MSA-core elements do not accumulate specifically in the upstream of mitosis-specific transcriptional genes. Mutations of the four MSA-core elements of the cyclin B gene, which encodes a central factor of the G2-to-M-phase transition, have resulted in the abolishment of transcription or permission of transcription even in the G1 phase. These results suggest that all four MSA-core elements located in the upstream region of cyclin B are involved in G2/M-phase-specific transcription in C. merolae; however, the nature of the involvement of MSA-core elements in G2/M-phase-specific transcription differed among the four elements. Thus, MSA-core-element-mediated G2/M-phase-specific transcription in C. merolae seems to be regulated by a complex mechanism.
KW - Cell cycle
KW - Cyanidioschyzon merolae
KW - Cyclin B
KW - G2/M-phase
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U2 - 10.1007/s10265-021-01334-z
DO - 10.1007/s10265-021-01334-z
M3 - Article
AN - SCOPUS:85111839534
SN - 0918-9440
VL - 134
SP - 1301
EP - 1310
JO - Journal of Plant Research
JF - Journal of Plant Research
IS - 6
ER -