Cis elements for transcriptional regulation of the human endothelin-A receptor gene

Jun Yamashita, Takaaki Yoshimasa, Hiroshi Arai, Junko Hiraoka, Kazuhiko Takaya, Yoshihiro Miyamoto, Yoshihiro Ogawa, Hiroshi Itoh, Kazuwa Nakao

研究成果: Article査読

3 被引用数 (Scopus)


To elucidate the transcriptional regulation of the endothelin-A receptor (ET-AR) gene, we defined the cis elements in the 5'-flanking region by functional analysis. Chimeric gene constructs were prepared using serial deletion mutants of a 5-kilobase (kb) fragment of the human ET-AR 5'-flanking region and the luciferase gene and were transfected into a rat vascular smooth-muscle cell line, A7r5. Deletion of the region between -2.0 kb and - 857 bases caused a twofold increase in luciferase activity, and deletion from - 137 bases to -53 bases reduced the activity by 50%. These results indicate the existence of a negative regulatory element and a positive regulatory element, respectively. The gel shift assay revealed the existence of nuclear proteins in A7r5 cells that bind to the region containing the positive regulatory element. Thus, we identified cis regulatory elements and nuclear factors that regulate the expression of the ET-AR gene.

ジャーナルJournal of Cardiovascular Pharmacology
出版ステータスPublished - 1995

ASJC Scopus subject areas

  • 薬理学
  • 循環器および心血管医学


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