TY - JOUR
T1 - Comparison of columns of chemically modified porous glass and silica in reversed-phase high-performance liquid chromatography of ginsenosides
AU - Kanazawa, Hideko
AU - Nagata, Yoshiko
AU - Kurosaki, Emi
AU - Matsushima, Yoshikazu
AU - Takai, Nobuharu
N1 - Funding Information:
pyranoseM. PG-ODS achieved a better separaACKNOWLEDGEMENTStion of the isomeric glycosides at C-20. The fact that the separation factors of the isomeric glycsoils-ides oThis work was supported in part by the Sciencen ica-ODS columns (80 andA 3)0w0e re similasur g-Research Promotion Fund of the Japan Private gests that the pore size did not affect the separationSchool Promotion Foundation and by Ise Chemical of these isomeric glycosides. Industries. We thank Professor I. Kitagawa of the
PY - 1993/2/19
Y1 - 1993/2/19
N2 - Reversed-phase high-performance liquid chromatograms of an extract of ginseng and mixtures of ginsenosides (ginseng saponins) on a number of columns of chemically modified porous glass (MPG, pore size 550 Å) and silica (pore size 80 and 300 Å) were compared. Although the retention behaviour of ginsenosides was similar on the columns examined, the capacity factors of ten ginsenosides on an octadecylsilyl-MPG (MPG-ODS) column were smaller than those on silica columns. It is concluded that the MPG-ODS column has a number of advantages over conventional silica-ODS columns for the chromatography of ginsenosides. These properties are attributable to the optimum pore size for the molecular size of the saponins on the one hand and to the narrow distribution range of the pore size on the other.
AB - Reversed-phase high-performance liquid chromatograms of an extract of ginseng and mixtures of ginsenosides (ginseng saponins) on a number of columns of chemically modified porous glass (MPG, pore size 550 Å) and silica (pore size 80 and 300 Å) were compared. Although the retention behaviour of ginsenosides was similar on the columns examined, the capacity factors of ten ginsenosides on an octadecylsilyl-MPG (MPG-ODS) column were smaller than those on silica columns. It is concluded that the MPG-ODS column has a number of advantages over conventional silica-ODS columns for the chromatography of ginsenosides. These properties are attributable to the optimum pore size for the molecular size of the saponins on the one hand and to the narrow distribution range of the pore size on the other.
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U2 - 10.1016/0021-9673(93)80028-7
DO - 10.1016/0021-9673(93)80028-7
M3 - Article
AN - SCOPUS:0027511398
SN - 0021-9673
VL - 632
SP - 79
EP - 85
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -