TY - JOUR
T1 - Construction of a New Drug and Agrochemical Candidates Screening Platform Utilizing Drug-Hypersensitive Fission Yeast to Discover Overlooked Natural Products
AU - Kimishima, Aoi
AU - Negami, Sota
AU - Honma, Sota
AU - Kawashima, Shigehiro A.
AU - Yashiroda, Yoko
AU - Fuji, Shin ichi
AU - Kojima, Hiroki
AU - Tokiwa, Toshiyuki
AU - Sugawara, Akihiro
AU - Ujie, Yukiko
AU - Abe, Akio
AU - Chinen, Takumi
AU - Yoshida, Minoru
AU - Usui, Takeo
AU - Asami, Yukihiro
N1 - Publisher Copyright:
© 2025 American Chemical Society and American Society of Pharmacognosy
PY - 2025/9/26
Y1 - 2025/9/26
N2 - Natural products exhibiting selective or preferential antifungal activity against fission yeast over budding yeast might contribute greatly to new discoveries in the life sciences and new drug and agrochemical development. However, it is difficult to discover new drug and agrochemical candidates in the fission yeast screening system due to its low drug sensitivity. In this study, we constructed a new antifungal drug and agrochemical candidate screening platform using a drug-hypersensitive fission yeast strain. We executed antifungal activity profiling of our natural compound and microbial libraries against drug-hypersensitive fission yeast and multidrug-sensitive budding yeast. Ultimately, we identified MS-347a as a promising agrochemical candidate due to its excellent activity against the rice blast fungus.
AB - Natural products exhibiting selective or preferential antifungal activity against fission yeast over budding yeast might contribute greatly to new discoveries in the life sciences and new drug and agrochemical development. However, it is difficult to discover new drug and agrochemical candidates in the fission yeast screening system due to its low drug sensitivity. In this study, we constructed a new antifungal drug and agrochemical candidate screening platform using a drug-hypersensitive fission yeast strain. We executed antifungal activity profiling of our natural compound and microbial libraries against drug-hypersensitive fission yeast and multidrug-sensitive budding yeast. Ultimately, we identified MS-347a as a promising agrochemical candidate due to its excellent activity against the rice blast fungus.
UR - https://www.scopus.com/pages/publications/105017329969
UR - https://www.scopus.com/pages/publications/105017329969#tab=citedBy
U2 - 10.1021/acs.jnatprod.5c00598
DO - 10.1021/acs.jnatprod.5c00598
M3 - Article
C2 - 40848261
AN - SCOPUS:105017329969
SN - 0163-3864
VL - 88
SP - 2090
EP - 2096
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 9
ER -