TY - GEN
T1 - Construction of vascular-mimetic tissue in a separable microchip
AU - Yamashita, T.
AU - Tanaka, Y.
AU - Sugii, Y.
AU - Mawatari, K.
AU - Kitamori, T.
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We here report the new culturing method of vascular cells using a separable microchip whose substrates can be combined and separated reversibly. This separable microchip allowed to culture smooth muscle cells (SMCs) under the medium perfusion, and to subsequently recover the cultured cells from microchannel only by reducing the temperature. This system has a potential to produce micro scale vascular-mimetic tissue consisting of endothelial cells (ECs) and smooth muscle cells (SMCs), and will be useful for the regeneration of various tissues with multilayered complex structures.
AB - We here report the new culturing method of vascular cells using a separable microchip whose substrates can be combined and separated reversibly. This separable microchip allowed to culture smooth muscle cells (SMCs) under the medium perfusion, and to subsequently recover the cultured cells from microchannel only by reducing the temperature. This system has a potential to produce micro scale vascular-mimetic tissue consisting of endothelial cells (ECs) and smooth muscle cells (SMCs), and will be useful for the regeneration of various tissues with multilayered complex structures.
KW - Separable microchip
KW - Surface modification
KW - Vascular tissue
UR - http://www.scopus.com/inward/record.url?scp=84884405597&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884405597&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884405597
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1316
EP - 1318
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -