TY - JOUR
T1 - Contribution of multidrug resistance-associated proteins (MRPs) to the release of prostanoids from A549 cells
AU - Furugen, Ayako
AU - Yamaguchi, Hiroaki
AU - Tanaka, Nobuaki
AU - Shiida, Narumi
AU - Ogura, Jiro
AU - Kobayashi, Masaki
AU - Iseki, Ken
PY - 2013
Y1 - 2013
N2 - Previous studies indicated that several members of the multidrug resistance-associated protein (MRP) family mediate the transport of prostanoids. However, theimportance of MRPs in the release process of prostanoids has not been fully elucidated. In this study, we investigated the contribution of MRPs, including MRP1, MRP2, and MRP4, to the release process of the prostanoids from human lung adenocarcinoma epithelial A549 cells. The extracellular levels of PGE2, PGF2α, and TXB2 (a metabolite of TXA2) were decreased by treatment with MRP inhibitors (dipyridamole, MK571, and probenecid). The studies using membrane vesicle suggest that the effects of the inhibitors were in part by inhibiting MRP4 function. The effects of knockdown of each MRP (MRP1, MRP2, and MRP4) were also investigated. The extracellular levels of PGE2 and PGF2α were significantly decreased after MRP4 knockdown. Our results suggest that MRPs including MRP4 contribute the release process of prostanoids in A549 cells.
AB - Previous studies indicated that several members of the multidrug resistance-associated protein (MRP) family mediate the transport of prostanoids. However, theimportance of MRPs in the release process of prostanoids has not been fully elucidated. In this study, we investigated the contribution of MRPs, including MRP1, MRP2, and MRP4, to the release process of the prostanoids from human lung adenocarcinoma epithelial A549 cells. The extracellular levels of PGE2, PGF2α, and TXB2 (a metabolite of TXA2) were decreased by treatment with MRP inhibitors (dipyridamole, MK571, and probenecid). The studies using membrane vesicle suggest that the effects of the inhibitors were in part by inhibiting MRP4 function. The effects of knockdown of each MRP (MRP1, MRP2, and MRP4) were also investigated. The extracellular levels of PGE2 and PGF2α were significantly decreased after MRP4 knockdown. Our results suggest that MRPs including MRP4 contribute the release process of prostanoids in A549 cells.
KW - Multidrug resistance-associated protein
KW - Prostaglandin E
KW - Prostaglandin F
KW - Prostanoid release
KW - Thromboxane A
UR - https://www.scopus.com/pages/publications/84885395889
UR - https://www.scopus.com/pages/publications/84885395889#tab=citedBy
U2 - 10.1016/j.prostaglandins.2013.08.002
DO - 10.1016/j.prostaglandins.2013.08.002
M3 - Article
C2 - 23994649
AN - SCOPUS:84885395889
SN - 1098-8823
VL - 106
SP - 37
EP - 44
JO - Prostaglandins and Other Lipid Mediators
JF - Prostaglandins and Other Lipid Mediators
IS - 1
ER -