Corneal endothelial changes following minor trauma

H. Fukami, R. A. Laing, K. Tsubota, K. Chiba, S. S. Oak

研究成果: Article査読

9 被引用数 (Scopus)


The healing processes that occur when corneal endothelial cells are subjected to only mild trauma are not known. To study these processes we have developed a system that enables only a few endothelial cells to be traumatized or destroyed under continuous specular microscopic observation in vitro. Experiments in which a small group of cells were traumatized by gentle wounding with a microglass tip produced an immediate and distinct dark area having the same size as the tip. Using this method we have produced and observed two types of wound by controlling the force of wounding. The first type of wound, produced by a gentle touch, recovered within 1 hr. The second type of wound, produced by moderate touch, took about 24 hr to recover completely from the trauma. In the second type of wound, we observed migration, elongation, coalescence and mitosis during the healing process. Histological examination revealed that in the first type of wound, the cells remained intact with no apparent damage seen by vital staining and light microscopy. For the second type of wound, the cells were completely missing although there was no apparent damage to Descemet's membrane.

ジャーナルInvestigative Ophthalmology and Visual Science
出版ステータスPublished - 1988

ASJC Scopus subject areas

  • 眼科学
  • 感覚系
  • 細胞および分子神経科学


「Corneal endothelial changes following minor trauma」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。