TY - JOUR
T1 - Cost-effective culture of human induced pluripotent stem cells using UV/ozone-modified culture plastics with reduction of cell-adhesive matrix coating
AU - Kasai, Kohei
AU - Tohyama, Shugo
AU - Suzuki, Hayato
AU - Tanosaki, Sho
AU - Fukuda, Keiichi
AU - Fujita, Jun
AU - Miyata, Shogo
N1 - Funding Information:
This work was partially supported by the Highway Program for Realization of Regenerative Medicine from Japan Agency for Medical Research and Development (AMED) (to K.F. 17bm054006h0007 ), the Translational Research Network Program from AMED (to S.M.), and NIMS microstructural characterization platform (NMCP) as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. A Grant-in-Aid for Scientific Research from the MEXT (to J.F., no. 19H03660 ). The authors are grateful to Dr. Hideo Iwai in NMCP for supporting ToF-SIMS measurements.
Funding Information:
This work was partially supported by the Highway Program for Realization of Regenerative Medicine from Japan Agency for Medical Research and Development (AMED) (to K.F. 17bm054006h0007), the Translational Research Network Program from AMED (to S.M.), and NIMS microstructural characterization platform (NMCP) as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. A Grant-in-Aid for Scientific Research from the MEXT (to J.F. no.19H03660). The authors are grateful to Dr. Hideo Iwai in NMCP for supporting ToF-SIMS measurements.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/6
Y1 - 2020/6
N2 - Human induced pluripotent stem cells (hiPSCs) are considered to be one of the most promising cell resources for regenerative medicine. HiPSCs usually maintain their pluripotency when they are cultured on feeder cell layers or are attached to a cell-adhesive extracellular matrix. In this study, we developed a culture system based on UV/ozone modification for conventional cell culture plastics to generate a suitable surface condition for hiPSCs. Time of flight secondary ion mass spectrometry (ToF-SIMS) was carried out to elucidate the relationship between hiPSC adhesion and UV/ozone irradiation-induced changes to surface chemistry of cell culture plastics. Cell culture plastics with modified surfaces enabled growth of a feeder-free hiPSC culture with markedly reduced cell-adhesive matrix coating. Our cell culture system using UV/ozone-modified cell culture plastics may produce clinically relevant hiPSCs at low costs, and can be easily scaled up in culture systems to produce a large number of hiPSCs.
AB - Human induced pluripotent stem cells (hiPSCs) are considered to be one of the most promising cell resources for regenerative medicine. HiPSCs usually maintain their pluripotency when they are cultured on feeder cell layers or are attached to a cell-adhesive extracellular matrix. In this study, we developed a culture system based on UV/ozone modification for conventional cell culture plastics to generate a suitable surface condition for hiPSCs. Time of flight secondary ion mass spectrometry (ToF-SIMS) was carried out to elucidate the relationship between hiPSC adhesion and UV/ozone irradiation-induced changes to surface chemistry of cell culture plastics. Cell culture plastics with modified surfaces enabled growth of a feeder-free hiPSC culture with markedly reduced cell-adhesive matrix coating. Our cell culture system using UV/ozone-modified cell culture plastics may produce clinically relevant hiPSCs at low costs, and can be easily scaled up in culture systems to produce a large number of hiPSCs.
KW - Cell adhesive matrix
KW - Cell culture plastics
KW - Human induced pluripotent stem cell
KW - Surface chemistry
KW - UV/ozone surface modification
UR - http://www.scopus.com/inward/record.url?scp=85081697006&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85081697006&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2020.110788
DO - 10.1016/j.msec.2020.110788
M3 - Article
C2 - 32279811
AN - SCOPUS:85081697006
SN - 0928-4931
VL - 111
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
M1 - 110788
ER -
