Cyclic GMP inhibits and shifts the activation curve of the delayed rectifier (I(K1)) of type I mammalian vestibular hair cells

O. Behrend, C. Schwark, T. Kunihiro, M. Strupp

研究成果: Article査読

34 被引用数 (Scopus)

抄録

NITRIC oxide (NO) plays a role in the modulation of the predominant potassium current of type I vestibular hair cells, a low-voltage activated current called I(K1). Since many effects of NO are mediated via cGMP, patch- clamp recordings were made to evaluate the effects of cGMP on I(K1). In whole-cell recordings 1 mM cGMP shifted V(half) of I(K1) by 15.0 ± 2.4 mV (n = 6) to more positive. In cell-attached 'multichannel recordings 1 mM 8- bromo-cGMP caused a reversible shift of V(half) by 13.8 ± 2.6 mV (n = 12) and in single channel recordings in the cell-attached configuration the open probability was reduced at -60 mV from 0.39 ± 0.14 to 0.08 ± 0.01. cGMP had no effect on excised inside-out patches, indicating that an intact cytosolic milieu with functioning phosphorylation cascades is necessary. cGMP seems to be an important second messenger which reduces the potassium conductance of vestibular hair cells.

本文言語English
ページ(範囲)2687-2690
ページ数4
ジャーナルNeuroReport
8
12
DOI
出版ステータスPublished - 1997 1月 1

ASJC Scopus subject areas

  • 神経科学一般

フィンガープリント

「Cyclic GMP inhibits and shifts the activation curve of the delayed rectifier (I(K1)) of type I mammalian vestibular hair cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル