Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte

Emiyu Ogawa, Sayaka Motohashi, Tsunenori Arai

研究成果: Conference contribution

抄録

We studied the myocardial cell necrosis measuring cell lethality with various albumin concentrations against oxidative stress by extracellular photosensitization reaction using talaporfin sodium. We have proposed to induce the photosensitization reaction in interstitial space of myocardium with short drug-light interval to realize the immediate cell damage by myocardial cell membrane for tachyarrhythmia treatment. To understand the effect of the particular extracellular photosensitization reaction in interstitial space, we measured the myocardial cell lethality 2 hours after the photosensitization reaction by WST assay varying the talaporfin sodium concentration, radiant exposure, and wide albumin concentration of 0-15 mg/ml. The cell lethality was decreased with albumin concentration increasing over 85 % in binding ratio between talaporfin sodium and albumin. The cell lethality didn’t change between 0-85% in binding ratio between talaporfin sodium and albumin. The calculated deposited energy to the talaporfin sodium solution was nearly constant of 8.4±1.6 J/well in average varying albumin concentration. We think the cell killing effect by the extracellular photosensitization reaction has a threshold between 85-100% in the binding ratio between talaporfin sodium and albumin in the case of the talaporfin sodium concentration of 40 μg/ml and radiant exposure of 0-40 J/cm2.

本文言語English
ホスト出版物のタイトルOptical Interactions with Tissue and Cells XXVI
編集者E. Duco Jansen
出版社SPIE
ISBN(電子版)9781628414110
DOI
出版ステータスPublished - 2014 1月 1
イベントOptical Interactions with Tissue and Cells XXVI - San Francisco, United States
継続期間: 2015 2月 82015 2月 10

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
9321
ISSN(印刷版)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXVI
国/地域United States
CitySan Francisco
Period15/2/815/2/10

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

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