TY - JOUR
T1 - Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli
AU - Mizushima, Tohru
AU - Tanino, Junko
AU - Miki, Takeyoshi
AU - Sekimizu, Kazuhisa
N1 - Funding Information:
We thank M. Ohara for comments on the manuscript. This work was supported in part by Grants in Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
PY - 1996/1/5
Y1 - 1996/1/5
N2 - We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitive dnaA mutants, including dnaA5 and dnaA46 mutants. The linking number of DNA prepared from dnaA mutants growing at 37°C was lower than that from wild type cells yet there was no significant difference when cells were grown at 28°C. Complementation analysis with a plasmid containing the wild type dnaA gene and phage P1-mediated transduction confirmed that mutations in the dnaA gene were responsible for the decrease in the linking number of DNA.
AB - We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitive dnaA mutants, including dnaA5 and dnaA46 mutants. The linking number of DNA prepared from dnaA mutants growing at 37°C was lower than that from wild type cells yet there was no significant difference when cells were grown at 28°C. Complementation analysis with a plasmid containing the wild type dnaA gene and phage P1-mediated transduction confirmed that mutations in the dnaA gene were responsible for the decrease in the linking number of DNA.
UR - http://www.scopus.com/inward/record.url?scp=0030062946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030062946&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0024
DO - 10.1006/bbrc.1996.0024
M3 - Article
C2 - 8573119
AN - SCOPUS:0030062946
SN - 0006-291X
VL - 218
SP - 137
EP - 141
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -