Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells

Yasumasa Hara; Midori A. Arai; Kanae Sakai; Naoki Ishikawa; Tohru Gonoi; Takashi Yaguchi; Masami Ishibashi

doi:10.1007/s11418-017-1161-y

Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of animal cells

Yasumasa Hara
, Midori A. Arai
, Kanae Sakai
, Naoki Ishikawa
, Tohru Gonoi
, Takashi Yaguchi
, Masami Ishibashi

研究成果: Article › 査読

21 被引用数 (Scopus)

抄録

A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.

本文言語	English
ページ（範囲）	280-289
ページ数	10
ジャーナル	Journal of Natural Medicines
巻	72
号	1
DOI	https://doi.org/10.1007/s11418-017-1161-y
出版ステータス	Published - 2018 1月 1
外部発表	はい

ASJC Scopus subject areas

分子医療

文献へのアクセス

10.1007/s11418-017-1161-y

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引用スタイル

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title = "Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells",

abstract = "A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a {\textquotedblleft}co-culture{\textquotedblright} in which the genus Nocardia was cultured in the presence of an animal cell line.",

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note = "Funding Information: Acknowledgments This study was supported by JSPS KAKENHI Grant Number 17H03992 and the Strategic Priority Research Promotion Program of Chiba University {\textquotedblleft}Phytochemical Plant Molecular Sciences{\textquotedblright}. Publisher Copyright: {\textcopyright} 2017, The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature.",

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AU - Ishikawa, Naoki

AU - Gonoi, Tohru

AU - Yaguchi, Takashi

AU - Ishibashi, Masami

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KW - Actinomycetes

KW - Amide

KW - Co-culture

KW - Nocardia

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