TY - GEN
T1 - Detecting and tracking multiple fluorescence spot in noisy DXT image sequence
AU - Sakakura, Reo
AU - Aoki, Yoshimitsu
AU - Shimizu, Keishi
AU - Oiki, Shigetoshi
PY - 2010
Y1 - 2010
N2 - Quantitative analysis of molecular structural change by means of Diffracted X-ray tracking (DXT) method requires tracking of dozens of bright spots in noisy image sequence. As the observation equipment upgrades, experimental data was cannot be analyzed manually with sufficient speed and accuracy. We propose automatic analysis method combining background estimation and frame-by-frame matching. We applied propose method to real DXT image data acquired for potassium ion channel structural changing analysis for evaluation. The result of experiments show that the method has high accuracy and be able to replace manual analysis.
AB - Quantitative analysis of molecular structural change by means of Diffracted X-ray tracking (DXT) method requires tracking of dozens of bright spots in noisy image sequence. As the observation equipment upgrades, experimental data was cannot be analyzed manually with sufficient speed and accuracy. We propose automatic analysis method combining background estimation and frame-by-frame matching. We applied propose method to real DXT image data acquired for potassium ion channel structural changing analysis for evaluation. The result of experiments show that the method has high accuracy and be able to replace manual analysis.
KW - Background etimation
KW - Component
KW - Fluorescence image sequence
KW - Kernel density estimation
KW - Multiple object tracking
KW - Occurrence probability
UR - http://www.scopus.com/inward/record.url?scp=78649252087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649252087&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:78649252087
SN - 9784907764364
T3 - Proceedings of the SICE Annual Conference
SP - 96
EP - 98
BT - Proceedings of SICE Annual Conference 2010, SICE 2010 - Final Program and Papers
PB - Society of Instrument and Control Engineers (SICE)
ER -