TY - JOUR
T1 - Determination of copper(II) chlorophyllin by reversed-phase high-performance liquid chromatography
AU - Inoue, H.
AU - Yamashita, H.
AU - Furuya, K.
AU - Nonomura, Y.
AU - Yoshioka, N.
AU - Lib, S.
N1 - Funding Information:
This work was partially supportedb y a Grant-in-Aid for scientific research, No. 06453071, from the Ministry of Education, Science and Culture, Japan.
PY - 1994/9/9
Y1 - 1994/9/9
N2 - Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper (II) chlorin e4, was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyllin in the concentration range of 0-30 μg cm-3 with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper(II) chlorin e4 were 3.5, 1.5, 3.3 and 1.4 ng cm-3 with relative standard deviations (n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin.
AB - Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper (II) chlorin e4, was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyllin in the concentration range of 0-30 μg cm-3 with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper(II) chlorin e4 were 3.5, 1.5, 3.3 and 1.4 ng cm-3 with relative standard deviations (n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin.
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U2 - 10.1016/0021-9673(94)80315-3
DO - 10.1016/0021-9673(94)80315-3
M3 - Article
AN - SCOPUS:0028038133
SN - 0021-9673
VL - 679
SP - 99
EP - 104
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
ER -