Determination of aminoglycoside antibiotics (AGs) in urine was investigated. Though AGs concentration in urine is usually determined by bioassay or HPLC, immunoassay is considered to be the best method due to its rapidity and convenience. Since determination of AGs in urine by immunoassay has not been investigated enough, we tried to determine gentamicin(GM), a kind of AGs, in urine by fluorescence polarization immunoassay (TDX)®. In consequence, while keeping its accuracy, we could determine GM in urine by the usual TDX method when the samples were diluted in the range of determination. We determined GM in 41 urine samples of 11 patients (infants) by TDX, HPLC and bioassay. Assay data by TDX agreed closely with those by HPLC and bioassay, and practical use of this method was confirmed. As it has become possible to determine urine concentration by TDX, it is easy to use urine concentration as a parameter in pharmacokinetics. This data can be utilized as useful information for administration protocol.
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