TY - JOUR
T1 - Determination of omeprazole and its metabolites in human plasma by liquid chromatography-mass spectrometry
AU - Kanazawa, Hideko
AU - Okada, Akiko
AU - Matsushima, Yoshikazu
AU - Yokota, Hiromitsu
AU - Okubo, Shigeo
AU - Mashige, Fumiko
AU - Nakahara, Kazuhiko
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/3/8
Y1 - 2002/3/8
N2 - Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 (CYP) 3A4 and CYP2C19, the genetic polymorphism of CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography-three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C18(50×2.0 mm I.D.) using acetonitrile-50 mM ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110°C and Shield temperature was 230°C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol.
AB - Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 (CYP) 3A4 and CYP2C19, the genetic polymorphism of CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography-three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C18(50×2.0 mm I.D.) using acetonitrile-50 mM ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110°C and Shield temperature was 230°C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol.
KW - Benzimidazoles
KW - Cytochromes
KW - Interfaces, LC-MS
KW - Omeprazole
KW - Sonic spray ionization
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U2 - 10.1016/S0021-9673(01)01508-4
DO - 10.1016/S0021-9673(01)01508-4
M3 - Article
C2 - 11999727
AN - SCOPUS:0037040556
SN - 0021-9673
VL - 949
SP - 1
EP - 9
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -
