TY - JOUR
T1 - Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry
AU - Kanazawa, Hideko
AU - Atsumi, Ryoko
AU - Matsushima, Yoshikazu
AU - Kizu, Junko
PY - 2000/2/18
Y1 - 2000/2/18
N2 - Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.
AB - Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.
KW - Caffeine
KW - Column switching
KW - Theophylline
UR - http://www.scopus.com/inward/record.url?scp=0033956667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033956667&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(99)00891-2
DO - 10.1016/S0021-9673(99)00891-2
M3 - Article
C2 - 10722065
AN - SCOPUS:0033956667
SN - 0021-9673
VL - 870
SP - 87
EP - 96
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -