@inproceedings{1f2f4990e5564e409f118a6aa5c770d5,
title = "Differentiation of multipotent DFAT cells into smooth muscle-like cells in 3d tubular microenvironment for tissue regeneration applications",
abstract = "This paper describes the differentiation of dedifferentiated fat (DFAT) cells cultured within alginate shell microfibers into smooth muscle-like cells. A microfluidic co-axial device was developed and used to fabricate DFAT cell fibers. DFAT cells embedded in extracellular matrix (ECM) composed of fibrin and collagen inside the alginate shell of microfibers were cultured for one week in control and differentiation induction media. Using such technique, we successfully cultured DFAT cells as 3D fibers and demonstrated differentiation of DFAT cells into smooth muscle-like cells. These findings suggest the use of DFAT 3D cell fibers as a promising and efficient approach for tissue regeneration.",
keywords = "3D culture, Alginate, DFAT, Regenerative medicine, Smooth muscle cells, Tissue engineering",
author = "Hsiao, \{Amy Y.\} and Teru Okitsu and Hiroaki Onoe and Mahiro Kiyosawa and Hiroki Teramae and Shintaroh Iwanaga and Shigenori Miura and Tomohiko Kazama and Taro Matsumoto and Shoji Takeuchi",
year = "2012",
month = jan,
day = "1",
language = "English",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "1150--1152",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}