Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Yohei Bamba; Tomoko Shofuda; Daisuke Kanematsu; Masahiro Nonaka; Mami Yamasaki; Hideyuki Okano; Yonehiro Kanemura

doi:10.1016/j.bbrc.2014.04.070

Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Yohei Bamba, Tomoko Shofuda, Daisuke Kanematsu, Masahiro Nonaka, Mami Yamasaki, Hideyuki Okano, Yonehiro Kanemura

生理学

研究成果: Article › 査読

14 被引用数 (Scopus)

抄録

Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.

本文言語	English
ページ（範囲）	683-688
ページ数	6
ジャーナル	Biochemical and Biophysical Research Communications
巻	447
号	4
DOI	https://doi.org/10.1016/j.bbrc.2014.04.070
出版ステータス	Published - 2014 5月 16

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

文献へのアクセス

10.1016/j.bbrc.2014.04.070

他のファイルとリンク

フィンガープリント

「Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Bamba, Y., Shofuda, T., Kanematsu, D., Nonaka, M., Yamasaki, M., Okano, H., & Kanemura, Y. (2014). Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochemical and Biophysical Research Communications, 447(4), 683-688. https://doi.org/10.1016/j.bbrc.2014.04.070

Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. / Bamba, Yohei; Shofuda, Tomoko; Kanematsu, Daisuke その他.
In: Biochemical and Biophysical Research Communications, Vol. 447, No. 4, 16.05.2014, p. 683-688.

研究成果: Article › 査読

Bamba, Y, Shofuda, T, Kanematsu, D, Nonaka, M, Yamasaki, M, Okano, H & Kanemura, Y 2014, 'Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics', Biochemical and Biophysical Research Communications, vol. 447, no. 4, pp. 683-688. https://doi.org/10.1016/j.bbrc.2014.04.070

Bamba Y, Shofuda T, Kanematsu D, Nonaka M, Yamasaki M, Okano H その他. Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochemical and Biophysical Research Communications. 2014 5月 16;447(4):683-688. doi: 10.1016/j.bbrc.2014.04.070

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abstract = "Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.",

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