@article{947c866e28c8491ab72f84802ae5f3f8,
title = "Dramatic improvement in genome assembly achieved using doubled-haploid genomes",
abstract = "Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 contig and scaffold of doubled-haploid individuals, respectively, compared to the wildtype, indicating that the use of a doubled-haploid genome aids in accurate genome analysis.",
author = "Hong Zhang and Engkong Tan and Yutaka Suzuki and Yusuke Hirose and Shigeharu Kinoshita and Hideyuki Okano and Jun Kudoh and Atsushi Shimizu and Kazuyoshi Saito and Shugo Watabe and Shuichi Asakawa",
note = "Funding Information: The authors would like to thank Y. Ito, T. Hara, S. Tsuchida, Y. Yamaguchi, Y. Watanabe, T. Shima, T. Watanabe, T. Setokuma, Y. Yoshino, T. Koda, and K. Sakae for technical support with the preliminary studies for this work and N. Mizuno and T. Mochizuki for technical support. We also thank T. Horiuchi, K. Imamura, T. Kikuchi, and M. Tosaka from the Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo and I. Koya and other members in the Collaborative Research Resources, Keio University School of Medicine for their aid in whole-genome sequencing. We thank A. Fujiwara for discussion of haploid fish use. This work was supported by JSPS KAKENHI, Grant Numbers 24248034 and 25640099.",
year = "2014",
month = oct,
day = "27",
doi = "10.1038/srep06780",
language = "English",
volume = "4",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}
