Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells

Masaki Kita, David St C. Black, Osamu Ohno, Kaoru Yamada, Hideo Kigoshi, Daisuke Uemura

研究成果: Article査読

17 被引用数 (Scopus)

抄録

(Chemical Equation Presented) The duck-billed platypus (Ornithorhynchus anatinus) is one of the few venomous Australian mammals. We previously found that its crude venom potently induces Ca2+ influx in human neuroblastoma IMR-32 cells. Guided by this bioassay, we identified 11 novel peptides, including the heptapeptide H-His-Asp-His-Pro-Asn-Pro-Arg-OH (1). Compounds 1-4 and 5-11 coincided with the 6-9 N-terminal residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP) and the 132-150 part of OvCNP precursor peptide, respectively. Heptapeptide 1, which is one of the primary components of the venom fluid ( 200 ng/μL), induced a significant increase in [Ca2+]i in IMR-32 cells at 75 μM. To the best of our knowledge, this is the first example of the isolation of the N-terminal linear fragments of CNPs in any mammal.

本文言語English
ページ(範囲)18038-18039
ページ数2
ジャーナルJournal of the American Chemical Society
131
50
DOI
出版ステータスPublished - 2009 12月 23
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 化学一般
  • 生化学
  • コロイド化学および表面化学

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