@inproceedings{2b61e9521d04412d9e77297c49336926,
title = "ECM-based Stretchable Microfluidic System for in vitro 3D Tissue Culture",
abstract = "We present an extracellular matrix (ECM)-based stretchable microfluidic system for in vitro three-dimensional (3D) tissue culture, which mimics the in vivo blood vessels. The target cells (ex. endothelial cells (ECs)) can be cultured with perfusion and stretch simultaneously in our proposed system. Our ECM (collagen)-based microfluidic device was fabricated by dissolving water-soluble sacrificial molds fabricated with 3D printer. We demonstrated perfusion and stretch of collagen microfluidic devices with culturing human umbilical vein endothelial cells (HUVECs), showing that the cultured cells were stretched along the expansion of the collagen microchannel. We believe that our collagen-based microfluidic stretch system could be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.",
keywords = "Collagen, Endothelial cell, Mechanical stress, Microfluidics, Tissue engineering",
author = "Azusa Shimizu and Goh, {Wei Huang} and Michinao Hashimoto and Shigenori Miura and Hiroaki Onoe",
note = "Funding Information: This work was partly supported by Keio University Special Grant-in-Aid for Innovative Collaborative Research Projects from Keio University, Japan. Publisher Copyright: {\textcopyright} 2019 IEEE.; 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII ; Conference date: 23-06-2019 Through 27-06-2019",
year = "2019",
month = jun,
doi = "10.1109/TRANSDUCERS.2019.8808795",
language = "English",
series = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "752--755",
booktitle = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",
}