Effect of Brazilian green propolis on oral pathogens and human periodontal fibroblasts

Hirotake Oda, Taneaki Nakagawa, Kosuke Maruyama, Yuzuru Dono, Hiroaki Katsuragi, Soh Sato

研究成果: Article査読

10 被引用数 (Scopus)


Objectives Plaque biofilm is the main etiological factor in dental caries and periodontitis. This study aimed to evaluate the effects of Brazilian green propolis (BGP), which has antibacterial and anti-inflammatory properties, on the growth of oral pathogens, and its cytotoxicity to adult human gingival fibroblasts (HGFs) and periodontal ligament fibroblasts (HPDLFs). Methods Streptococcus mutans, Streptococcus sanguinis, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were used to evaluate the antibacterial action of BGP. Media containing different concentrations of BGP were prepared, and their effect on bacterial growth was determined by measuring their optical density. The cytotoxicity of BGP to HGFs and HPDLFs was determined by measuring the proliferation and viability of cells with the Alamar Blue assay. Results No significant growth of P. gingivalis, S. mutans, or S. sanguinis was observed when exposed to concentrations of BGP above 2000 μg/ml, but growth of A. actinomycetemcomitans was not affected at this concentration. Growth of P. gingivalis and S. sanguinis was significantly inhibited when exposed to BGP at a concentration of 100 μg/ml. Growth of S. mutans was significantly inhibited in medium containing BGP at 500 μg/ml. Cellular viability (LD50) of HGFs and HPDLFs was not observed at 2000 μg/ml BGP. Conclusions These results suggest that BGP has antibacterial properties but is not significantly cytotoxic to periodontal fibroblasts.

ジャーナルJournal of Oral Biosciences
出版ステータスPublished - 2016 5月 1

ASJC Scopus subject areas

  • 医学(その他)
  • 生化学、遺伝学、分子生物学一般
  • 歯学一般


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