Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow, in vivo, and shear stress affects the intracellular calcium ([Ca2+]1), which initiates cellular responses. Although shear-stress induced [Ca2+]1 responses shortly (within 10 min after initiation of flow have been studied, the effects of long-term exposure (24 hr) of shear stress on [Ca2+]1 responses have not. Here, we studied the effect of long-term exposure of shear stress on [Ca2+]1 responses in cultured ECs by using a confocal laser microscope and calcium indicator (Calcium Green-1/ AM). At the initiation of shear stress of 2 Pa (0 hr), 27% of the cells exhibited [Ca2+]1 responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca2+]1 responses in cultured ECs.
|ジャーナル||JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing|
|出版ステータス||Published - 2003 12月|
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