TY - GEN
T1 - EFFECT OF SHEAR STRESS ON MITOCHONDRIAL MEMBRANE POTENTIAL OF CULTURED ENDOTHELIAL CELLS
AU - Kudo, Susumu
AU - Morigaki, Ryoma
AU - Ikeda, Mariko
AU - Oka, Kotaro
AU - Tanishita, Kazuo
N1 - Funding Information:
r.ndothelial shape and functions are influenced by external mechanical stimulus (low or high shear stress) {Wang et al.. 1991). So. the shear dependence of mitochondrial A'l'P synthesis function ma\ play a ke\ role for other cell functions. ACKNOWLEDGEMENTS This study was partially supported b> a (irant-in-Aid for General Scientific Research (06452187) from .lapan’s Ministiy of Education. Science and Culture, and the Science Research Promotion Eund in Japan. REFERENCES Arisaka. T.. Mitsumata. M.. Kawasumi. M.. Tohjima. T.. Hirose. S..and Yoshida.Y.. 1995. “l-TTect of Shear Stress on CiEcosaminoglycan Sxnthesis in Vascular Endothelial Cells ,Ann. .\ .lead. Sei., \ol. 748. pp.543-554. Dewe\, C.E. Jr.. Bussolari. S.R.. Gimbrone. M..A. Jr., and Davies. P.T.. 1981, "The Dxnamic Response of Vascular Endothelial Cells to Fluid Shear Stress ' . ,7 Biomech. Eiigr.. vol. 103. pp. 177-185. Levesque. M..I. and Nerem. R.M.. 1985. “The Elongation and Orientation of Culttired llndothelial Cells in Response to Shear Stress .7. Biomech. Engr.. vol. 107. pp.341-347. Reers. M.. Smith. W.T.. and Chen. B.T.. 1991 ‘J-Aggregate Formation of a Carbocyanine as a Quantitative Fluorescent Indicator of Membrane Potential” liiocheniisirv. vol.30, pp.4480-4486 Reers. M.. Smiley. F.S.. Mottola-Hartshorn. C.. Chen. A.. Lin. M.. and Chen. B.l... 1995 “Mitochondrial Membrane Potential Monitored bv .IC-1 Ove " Methods Enzymoi.. vol.260. pp.406-417. Smilev. T.S.. Reers. M.. Mottola-Hartshorn. C.. Lin. M.. Chen. A.. Smith. W.L.. Steele. D.O.. and Chen. B.L.. 1991 “Intracellular heterogeneitv in mitochondrial membrane potentials revealevl hv a J-aggregate-forming lipophilic cation JC- 1 l>roc \alL AcmI. Sei. I 'S.i. vol.88. pp.3671-3675. Wang. Okano. M.. and '(’osliida. M.. 1991 "bllraslruclure of endothelial cells and lipid deposition on the How dividers of branchiocephalie and left subclavian arterial bifurcation of the rabbit tiorta .J. .Ipn. . Itlieroxcler. Soc. vol. 19. pp. 1089-1 100.
Publisher Copyright:
© 1997 American Society of Mechanical Engineers (ASME). All rights reserved.
PY - 1997
Y1 - 1997
N2 - Endothelial cells line on the inner surface of the vascular is continuously exposed to shear stress by a blood flow in vivo. It is known that shear stress influences energy-dependent physical (Dewey et al., 1981, Levesque et al., 1985) and biochemical characteristics of endothelial cells (Arisaka et al., 1995). We, therefore, evaluated mitochondrial ATP synthesis activity by a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe, 5.5', 6.6'-tetrachloro-1.1',3,3'-tetrathylbenzimidazolocarbocyananine iodide (JC-1), when shear stress was loaded over cultured endothelial cells.
AB - Endothelial cells line on the inner surface of the vascular is continuously exposed to shear stress by a blood flow in vivo. It is known that shear stress influences energy-dependent physical (Dewey et al., 1981, Levesque et al., 1985) and biochemical characteristics of endothelial cells (Arisaka et al., 1995). We, therefore, evaluated mitochondrial ATP synthesis activity by a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe, 5.5', 6.6'-tetrachloro-1.1',3,3'-tetrathylbenzimidazolocarbocyananine iodide (JC-1), when shear stress was loaded over cultured endothelial cells.
UR - https://www.scopus.com/pages/publications/85126959778
UR - https://www.scopus.com/pages/publications/85126959778#tab=citedBy
U2 - 10.1115/IMECE1997-0229
DO - 10.1115/IMECE1997-0229
M3 - Conference contribution
AN - SCOPUS:85126959778
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
SP - 63
EP - 64
BT - Advances in Bioengineering
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 1997 International Mechanical Engineering Congress and Exposition, IMECE 1997 - Advances in Bioengineering
Y2 - 16 November 1997 through 21 November 1997
ER -