TY - JOUR
T1 - Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases
AU - Aketa, Naohiko
AU - Yamaguchi, Takefumi
AU - Asato, Teru
AU - Yagi-Yaguchi, Yukari
AU - Suzuki, Terumasa
AU - Higa, Kazunari
AU - Kurihara, Toshihide
AU - Satake, Yoshiyuki
AU - Tsubota, Kazuo
AU - Shimazaki, Jun
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/12
Y1 - 2017/12
N2 - Purpose To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. Design Prospective consecutive case series. Methods This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays. Results The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P =.001; IL-10: 1.68 ± 0.04, P =.0006; IL-17A: 3.7 ± 0.2, P =.008; GM-CSF: 2.7 ± 0.3, P =.007; E-selectin: 2093 ± 37, P =.0001; P-selectin: 3658 ± 137, P =.0001; sICAM-1: 1397 ± 119, P =.008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P =.0077; IL-17A: 4.1 ± 0.7, P =.034; E-selectin: 2439 ± 302, P =.039; and P-selectin: 5673 ± 1553, P =.017). Conclusions Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.
AB - Purpose To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. Design Prospective consecutive case series. Methods This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays. Results The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P =.001; IL-10: 1.68 ± 0.04, P =.0006; IL-17A: 3.7 ± 0.2, P =.008; GM-CSF: 2.7 ± 0.3, P =.007; E-selectin: 2093 ± 37, P =.0001; P-selectin: 3658 ± 137, P =.0001; sICAM-1: 1397 ± 119, P =.008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P =.0077; IL-17A: 4.1 ± 0.7, P =.034; E-selectin: 2439 ± 302, P =.039; and P-selectin: 5673 ± 1553, P =.017). Conclusions Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.
UR - https://www.scopus.com/pages/publications/85031823541
UR - https://www.scopus.com/pages/publications/85031823541#tab=citedBy
U2 - 10.1016/j.ajo.2017.09.029
DO - 10.1016/j.ajo.2017.09.029
M3 - Article
C2 - 28988898
AN - SCOPUS:85031823541
SN - 0002-9394
VL - 184
SP - 42
EP - 51
JO - American Journal of Ophthalmology
JF - American Journal of Ophthalmology
ER -