TY - JOUR
T1 - Endothelin in human plasma and culture medium of aortic endothelial cells-Detection and characterization with radioimmunoassay using monoclonal antibody
AU - Saito, Yoshihiko
AU - Nakao, Kazuwa
AU - Itoh, Hiroshi
AU - Yamada, Takayuki
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Hosoda, Kiminori
AU - Shirakami, Gotaro
AU - Suga, Shin ichi
AU - Jougasaki, Michihisa
AU - Morichika, Shoko
AU - Imura, Hiroo
N1 - Funding Information:
ACKNOWLEDGMENTSW: e thank Ms. Hiroko Fumon, Ms. Atsuko Furu and Ms. Naomi Sako for their excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1989, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-1 and 63C-2) from the Japanese Ministry of Health Welfare.
PY - 1989/5/30
Y1 - 1989/5/30
N2 - We have developed monoclonal (KY-ET-1-I) and polyclonal (ET-F5) antibodies against endothelin-1 (ET-1) and established sensitive radioimmunoassays (RIAs) with different specificities. The RIA with KY-ET-1-I detected ET-1, ET-2 and ET-3, while the RIA with ET-F5 recognized ET-3 very weakly. Using these RIAs, we have investigated the concentration and molecular forms of ET-1-like immunoreactivity (-LI) in culture medium of bovine aortic endothelial cells and human plasma. Culture medium of endothelial cells contained two major components compatible with big ET and ET-1. ET-1-LI was also detected in human plasma. ET-1-LI in human plasma consisted of apparent two components, the small molecular form emerging at the position of ET-1 and the large form with the peak eluting at the preceding fraction of the elution position of big ET. The concentration of the small form of ET in human plasma was about 5 pg/ml.
AB - We have developed monoclonal (KY-ET-1-I) and polyclonal (ET-F5) antibodies against endothelin-1 (ET-1) and established sensitive radioimmunoassays (RIAs) with different specificities. The RIA with KY-ET-1-I detected ET-1, ET-2 and ET-3, while the RIA with ET-F5 recognized ET-3 very weakly. Using these RIAs, we have investigated the concentration and molecular forms of ET-1-like immunoreactivity (-LI) in culture medium of bovine aortic endothelial cells and human plasma. Culture medium of endothelial cells contained two major components compatible with big ET and ET-1. ET-1-LI was also detected in human plasma. ET-1-LI in human plasma consisted of apparent two components, the small molecular form emerging at the position of ET-1 and the large form with the peak eluting at the preceding fraction of the elution position of big ET. The concentration of the small form of ET in human plasma was about 5 pg/ml.
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U2 - 10.1016/0006-291X(89)91599-4
DO - 10.1016/0006-291X(89)91599-4
M3 - Article
C2 - 2658998
AN - SCOPUS:0024339817
SN - 0006-291X
VL - 161
SP - 320
EP - 326
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -