Epidermal growth factor receptor-dependent cytotoxic effect of anti-EGFR antibody-ribonuclease conjugate on human cancer cells

Tatsushi Suwa, Masakazu Ueda, Hiromitsu Jinno, Soji Ozawa, Yuko Kitagawa, Nobutoshi Ando, Masaki Kitajima

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We have conjugated the murine monoclonal antibody (528) against the human epidermal growth factor receptor (EGFR) to mammalian pancreatic ribonuclease (RNase) via N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP) and 2-iminothiolene (2-IT). The conjugate showed dose-dependent cytotoxicity against EGFR-producing squamous cancer cells (A431, TE8, TE5, Ca9-22) and no detectable cytotoxicity against EGFR-deficient small-cell lung cancer cells (H69). The cytotoxicity of the conjugate was positively correlated with the EGFR numbers of each cell line. The addition of excess 528 antibody to the medium protected A431 cells from the conjugate cytotoxicity. This immuno-conjugate might be useful for targeted treatment of squamous cell carcinomas hyperexpressing EGFR.

本文言語English
ページ(範囲)4161-4165
ページ数5
ジャーナルAnticancer research
19
5 B
出版ステータスPublished - 1999

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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