Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

Chisato Watanabe; Hirotoshi Shibuya; Yusuke Ichiyama; Eiichi Okamura; Setsuko Tsukiyama-Fujii; Tomoyuki Tsukiyama; Shoma Matsumoto; Jun Matsushita; Takuya Azami; Yoshiaki Kubota; Masahito Ohji; Fumihiro Sugiyama; Satoru Takahashi; Seiya Mizuno; Masaru Tamura; Ken Ichi Mizutani; Masatsugu Ema

doi:10.3390/life12111730

Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

Chisato Watanabe, Hirotoshi Shibuya, Yusuke Ichiyama, Eiichi Okamura, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama, Shoma Matsumoto, Jun Matsushita, Takuya Azami, Yoshiaki Kubota, Masahito Ohji, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno, Masaru Tamura, Ken Ichi Mizutani, Masatsugu Ema

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Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice.

本文言語	English
論文番号	1730
ジャーナル	Life
巻	12
号	11
DOI	https://doi.org/10.3390/life12111730
出版ステータス	Published - 2022 11月

ASJC Scopus subject areas

生態、進化、行動および分類学
生化学、遺伝学、分子生物学（全般）
宇宙惑星科学
古生物学

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Watanabe, C., Shibuya, H., Ichiyama, Y., Okamura, E., Tsukiyama-Fujii, S., Tsukiyama, T., Matsumoto, S., Matsushita, J., Azami, T., Kubota, Y., Ohji, M., Sugiyama, F., Takahashi, S., Mizuno, S., Tamura, M., Mizutani, K. I., & Ema, M. (2022). Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice. Life, 12(11), Article 1730. https://doi.org/10.3390/life12111730

Watanabe, C, Shibuya, H, Ichiyama, Y, Okamura, E, Tsukiyama-Fujii, S, Tsukiyama, T, Matsumoto, S, Matsushita, J, Azami, T, Kubota, Y, Ohji, M, Sugiyama, F, Takahashi, S, Mizuno, S, Tamura, M, Mizutani, KI & Ema, M 2022, 'Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice', Life, vol. 12, no. 11, 1730. https://doi.org/10.3390/life12111730

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title = "Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice",

abstract = "Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice.",

keywords = "Exoc3l2, cardiovascular development, exocyst complex, exocyst complex component 3, hemorrhage, knock-out mouse",

author = "Chisato Watanabe and Hirotoshi Shibuya and Yusuke Ichiyama and Eiichi Okamura and Setsuko Tsukiyama-Fujii and Tomoyuki Tsukiyama and Shoma Matsumoto and Jun Matsushita and Takuya Azami and Yoshiaki Kubota and Masahito Ohji and Fumihiro Sugiyama and Satoru Takahashi and Seiya Mizuno and Masaru Tamura and Mizutani, {Ken Ichi} and Masatsugu Ema",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research (KAKENHI) (JP18H02363) to M.E. and Grants-in-Aid for JSPS Fellows (21J15321) to C.W. Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = nov,

doi = "10.3390/life12111730",

language = "English",

volume = "12",

journal = "Life",

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T1 - Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

AU - Watanabe, Chisato

AU - Shibuya, Hirotoshi

AU - Ichiyama, Yusuke

AU - Okamura, Eiichi

AU - Tsukiyama-Fujii, Setsuko

AU - Tsukiyama, Tomoyuki

AU - Matsumoto, Shoma

AU - Matsushita, Jun

AU - Azami, Takuya

AU - Kubota, Yoshiaki

AU - Ohji, Masahito

AU - Sugiyama, Fumihiro

AU - Takahashi, Satoru

AU - Mizuno, Seiya

AU - Tamura, Masaru

AU - Mizutani, Ken Ichi

AU - Ema, Masatsugu

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research (KAKENHI) (JP18H02363) to M.E. and Grants-in-Aid for JSPS Fellows (21J15321) to C.W. Publisher Copyright: © 2022 by the authors.

PY - 2022/11

Y1 - 2022/11

N2 - Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice.

AB - Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice.

KW - Exoc3l2

KW - cardiovascular development

KW - exocyst complex

KW - exocyst complex component 3

KW - hemorrhage

KW - knock-out mouse

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SN - 0024-3019

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JO - Life

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ER -

Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

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