Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito; Toshihide Muramatsu; Hidetsugu Saito

doi:10.1016/j.xpro.2019.100009

Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito, Toshihide Muramatsu, Hidetsugu Saito

薬学科

研究成果: Article › 査読

5 被引用数 (Scopus)

抄録

This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.

本文言語	English
論文番号	100009
ジャーナル	STAR Protocols
巻	1
号	1
DOI	https://doi.org/10.1016/j.xpro.2019.100009
出版ステータス	Published - 2020 6月 19

ASJC Scopus subject areas

生化学、遺伝学、分子生物学（全般）
神経科学（全般）
免疫学および微生物学（全般）

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.xpro.2019.100009

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Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

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ASJC Scopus subject areas

UN SDG

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