Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

Hirohisa Tajima; Takako Niikura; Yuichi Hashimoto; Yuko Ito; Yoshiko Kita; Kenzo Terashita; Kazuto Yamazaki; Atsuo Koto; Sadakazu Aiso; Ikuo Nishimoto

doi:10.1016/S0304-3940(02)00199-4

Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

Hirohisa Tajima, Takako Niikura, Yuichi Hashimoto, Yuko Ito, Yoshiko Kita, Kenzo Terashita, Kazuto Yamazaki, Atsuo Koto, Sadakazu Aiso, Ikuo Nishimoto

研究成果: Article › 査読

127 被引用数 (Scopus)

抄録

An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

本文言語	English
ページ（範囲）	227-231
ページ数	5
ジャーナル	Neuroscience Letters
巻	324
号	3
DOI	https://doi.org/10.1016/S0304-3940(02)00199-4
出版ステータス	Published - 2002 5月 24
外部発表	はい

ASJC Scopus subject areas

神経科学一般

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/S0304-3940(02)00199-4

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title = "Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults",

abstract = "An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.",

keywords = "Alzheimer's disease, Humanin, Humanin mRNA, Humanin peptide, In vivo expression, Mitochondrial 16S ribosomal RNA with a polyA tail, Neuronal death, Rescue factor",

author = "Hirohisa Tajima and Takako Niikura and Yuichi Hashimoto and Yuko Ito and Yoshiko Kita and Kenzo Terashita and Kazuto Yamazaki and Atsuo Koto and Sadakazu Aiso and Ikuo Nishimoto",

note = "Funding Information: The authors would like to thank Dr Masaki Kitajima and Mrs Yumi and Mr Yoshiomi Tamai for support; Dr Jun-ichi Hayashi for ρ 0 cells; and Ms Takako Hiraki, Dr Dovie Wylie, and Ms Kazumi Nishihara for assistance. This work was supported in part by grants from Chugai Pharmaceuticals, Japan Foundation for Neuroscience and Mental Health, Ono Medical Research Foundation, and the Ministry of Education, Culture, Sports, Science, and Technology of Japan.",

year = "2002",

month = may,

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doi = "10.1016/S0304-3940(02)00199-4",

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T1 - Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

AU - Tajima, Hirohisa

AU - Niikura, Takako

AU - Hashimoto, Yuichi

AU - Ito, Yuko

AU - Kita, Yoshiko

AU - Terashita, Kenzo

AU - Yamazaki, Kazuto

AU - Koto, Atsuo

AU - Aiso, Sadakazu

AU - Nishimoto, Ikuo

N1 - Funding Information: The authors would like to thank Dr Masaki Kitajima and Mrs Yumi and Mr Yoshiomi Tamai for support; Dr Jun-ichi Hayashi for ρ 0 cells; and Ms Takako Hiraki, Dr Dovie Wylie, and Ms Kazumi Nishihara for assistance. This work was supported in part by grants from Chugai Pharmaceuticals, Japan Foundation for Neuroscience and Mental Health, Ono Medical Research Foundation, and the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

PY - 2002/5/24

Y1 - 2002/5/24

N2 - An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

AB - An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

KW - Alzheimer's disease

KW - Humanin

KW - Humanin mRNA

KW - Humanin peptide

KW - In vivo expression

KW - Mitochondrial 16S ribosomal RNA with a polyA tail

KW - Neuronal death

KW - Rescue factor

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AN - SCOPUS:0037166058

SN - 0304-3940

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JO - Neuroscience Letters

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Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

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ASJC Scopus subject areas

UN SDG

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