TY - JOUR
T1 - Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (114/A10), in the megakaryocytic lineage
AU - Duan, Yun
AU - Naruse, Takeshi
AU - Nakamura, Mitsuru
AU - Yamaguchi, Yuji
AU - Kawashima, Toshiyuki
AU - Morikawa, Yoshihiro
AU - Kitamura, Toshio
AU - Suda, Toshio
N1 - Funding Information:
This work was supported by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan. We thank Drs. O. Saka-moto, H. Hirao, K. Hayakawa, and H. Osawa for collaboration in this work.
PY - 1998/12/18
Y1 - 1998/12/18
N2 - To analyze the functions of molecules expressed in hemopoietic progenitor cells, we obtained several monoclonal antibodies by immunizing Wistar rats with antigens from a murine immature leukemic cell line, DA-1. Here, we characterize one antibody designated NJ-1, which recognizes a 145-kd molecule, and identify the cDNA encoding the NJ-1 antigen by retrovirus-mediated expression cloning. Sequence analysis of the cDNA reveals that it is identical to a previously reported cDNA encoding a surface molecule of 573 amino acids recognized by monoclonal antibody 114/A10. Our studies show that expression of NJ-1 antigen is upregulated in a murine megakaryoblastic cell line, L8057, when it differentiates into a megakaryocytic lineage in response to 12-O-tetradecanoyl phorbol-13-acetate. Overexpression of NJ-1 antigen in L8057 cells inhibits cell adhesion to fibronectin, suggesting that it may act as a negative regulator of cell adhesion in the megakaryocytic lineage.
AB - To analyze the functions of molecules expressed in hemopoietic progenitor cells, we obtained several monoclonal antibodies by immunizing Wistar rats with antigens from a murine immature leukemic cell line, DA-1. Here, we characterize one antibody designated NJ-1, which recognizes a 145-kd molecule, and identify the cDNA encoding the NJ-1 antigen by retrovirus-mediated expression cloning. Sequence analysis of the cDNA reveals that it is identical to a previously reported cDNA encoding a surface molecule of 573 amino acids recognized by monoclonal antibody 114/A10. Our studies show that expression of NJ-1 antigen is upregulated in a murine megakaryoblastic cell line, L8057, when it differentiates into a megakaryocytic lineage in response to 12-O-tetradecanoyl phorbol-13-acetate. Overexpression of NJ-1 antigen in L8057 cells inhibits cell adhesion to fibronectin, suggesting that it may act as a negative regulator of cell adhesion in the megakaryocytic lineage.
KW - 114/A10
KW - Cell adhesion
KW - Hemopoietic progenitors
KW - Megakaryocytes
KW - NJ-1
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U2 - 10.1006/bbrc.1998.9700
DO - 10.1006/bbrc.1998.9700
M3 - Article
C2 - 9878549
AN - SCOPUS:0032545337
SN - 0006-291X
VL - 253
SP - 401
EP - 406
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -