Expression and localization of the water channels in human autosomal dominant polycystic kidney disease

Matsahiko Hayashi, Yasuyoshi Yamaji, Toshiaki Monkawa, Tadashi Yoshida, Hirohiko Tsuganezawa, Hiroyuki Sasamura, Waichi Kitajima, Sei Sasaki, Kennichi Ishibashi, Fumiaki Maurmo, Takao Saruta

研究成果: Article査読

19 被引用数 (Scopus)


To characterize the cyst-lining cells in human autosomal dominant polycystic kidney disease (ADPKD), we performed immunohistological studies with specific antibodies against human aquaporin-2 (AQP-2, the vasopressin-regulated water channel) and aquaporin-3 (AQP-3), which are expressed only in collecting duct cells in the normal kidney. The polycystic kidney samples were obtained from 2 hemodialysis patient at uninephrectomy. Immunohistochemical studies revealed two types of staining of cyst-lining cells. Approximately 30% of all the cysts were simultaneously immunostained by both antibodies. Among these AQP-positive cysts, more than 90% of the cysts were intensely stained, with well-polarized localization of AQP-2 and AQP-3. In fewer than 10% of AQP-positive cysts, by contrast, immunostaining for AQP-2 and AQP-3 was faint and no clearly polarized localization of the channels was observed. We examined the immunostaining in further detail by electron microscopy. Staining specific for AQP-2 was mainly observed in the apical membrane of cyst-lining cells. Moreover, staining specific for AQP-3 was observed in all of the AQP-2-positive cysts. It appeared unlikely that the variations in immunostaining observed under the light microscope had been induced by total disruption of water-channel polarity. The present study suggests that about 30% of the cysts in our cases of ADPKD were derived from the collecting duct cells and that the cyst-lining cells were well differentiated in terms of AQP expression.

出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 腎臓病学
  • 泌尿器学
  • 生理学(医学)
  • 生理学


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