TY - GEN
T1 - Fabrication method to a high resolution control in the space of cell culturing environment with microfluidic system
AU - Hiraiwa, Takumi
AU - Kimura, Tadamasa
AU - Takenaka, Yuma
AU - Tanamoto, Ryo
AU - Ota, Hiroki
AU - Kimura, Hiroshi
AU - Taguchi, Yoshihiro
AU - Miki, Norihisa
AU - Matsumoto, Yoshinori
AU - Oka, Kotaro
AU - Funahashi, Akira
AU - Hiroi, Noriko
PY - 2014/1/1
Y1 - 2014/1/1
N2 - This paper describes the fabrication and the evaluation of a reusable Cell Culturing Device, designed for a single cell and cellular networks analysis. This is the first success of combination of Microcontact Printing (mCP) and Vacuum Device. This combination has following advantages; (1) cells stay within the micropatterns for long enough duration to achieve local activation of cells or cellular networks (more than 24 h), (2) the displacement distance of laminar flow from the interface of two fluids in Cell Culturing Device keeps smaller than the diameter of a cell, (3) all components of our device except micropatterned substrate are reusable for further analyses. The success of the combination of above techniques provides a controllable environment for the local activation of a single cell and cellular networks. Our device allows to exhibit the different responses induced with the various conditions in a single observation sight at exactly the same time point.
AB - This paper describes the fabrication and the evaluation of a reusable Cell Culturing Device, designed for a single cell and cellular networks analysis. This is the first success of combination of Microcontact Printing (mCP) and Vacuum Device. This combination has following advantages; (1) cells stay within the micropatterns for long enough duration to achieve local activation of cells or cellular networks (more than 24 h), (2) the displacement distance of laminar flow from the interface of two fluids in Cell Culturing Device keeps smaller than the diameter of a cell, (3) all components of our device except micropatterned substrate are reusable for further analyses. The success of the combination of above techniques provides a controllable environment for the local activation of a single cell and cellular networks. Our device allows to exhibit the different responses induced with the various conditions in a single observation sight at exactly the same time point.
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U2 - 10.1109/MEMSYS.2014.6765626
DO - 10.1109/MEMSYS.2014.6765626
M3 - Conference contribution
AN - SCOPUS:84898960439
SN - 9781479935086
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 264
EP - 267
BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Y2 - 26 January 2014 through 30 January 2014
ER -