Femtosecond laser-processed biodegradable porous membranes for cultivation and transplantation of three-dimensional skin substitutes

Yasuyuki Tsunoi, Izumi Takayama, Naonari Kondo, Yo Nagano, Hiromi Miyazaki, Taiichiro Ida, Mitsuru Akashi, Mitsuhiro Terakawa, Shunichi Sato

研究成果: Conference contribution

抄録

We previously developed a pre-vascularized three-dimensional (3D) cultured human skin model, for which cells were cultured on a porous polymer membrane to supply a culture medium to the tissue. The model was transplanted onto a full-thickness skin defect in a mouse, showing the efficacy as a skin graft. However, there were difficulties in separating the cultured skins from the membranes, as well as in handling the separated soft skins for transplantation. To solve these problems, we recently developed biodegradable porous membranes that enable skin grafting together with the membranes. Poly (lactic-co-glycolic acid) (PLGA) thin films were irradiated with femtosecond laser pulses to create micro through-holes to produce porous membranes. The membrane showed complete decomposition in the mouse subcutaneous tissue within 35 days after implantation. Three-dimensional skins cultured on the membranes were then transplanted together with the membranes onto skin defects in mice, showing reepithelization in the grafted tissues. However, decomposition of the membrane was limited at the early-stage post-transplantation, and insufficient engraftment was observed in some cases. In this study, we increased the hole density of the PLGA membranes to improve their decomposition rate in tissue. We observed that the membrane was completely decomposed in the mouse subcutaneous tissue within 24 days after implantation. On the membranes, 3D skins containing vascular networks were cultivated. However, we encountered another problem that the mechanical strength of the membranes decreased with the increased hole density; some membranes were torn during skin cultivation. Considering this, we plan to further optimize the membrane conditions next.

本文言語English
ホスト出版物のタイトルPhotonics in Dermatology and Plastic Surgery 2024
編集者Haishan Zeng, Milind Rajadhyaksha
出版社SPIE
ISBN(電子版)9781510668911
DOI
出版ステータスPublished - 2024
イベントPhotonics in Dermatology and Plastic Surgery 2024 - San Francisco, United States
継続期間: 2024 1月 272024 1月 28

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
12816
ISSN(印刷版)1605-7422

Conference

ConferencePhotonics in Dermatology and Plastic Surgery 2024
国/地域United States
CitySan Francisco
Period24/1/2724/1/28

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 原子分子物理学および光学
  • 生体材料
  • 放射線学、核医学およびイメージング

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