Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells

Atsushi Yoshioka, Ryuichi Okamoto, Shigeru Oshima, Junko Akiyama, Kiichiro Tsuchiya, Tetsuya Nakamura, Takanori Kanai, Mamoru Watanabe

研究成果: Article査読

9 被引用数 (Scopus)


IL-7 is a cytokine, which regulates development, maintenance and proliferation of T lymphocytes within the human immune system. Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close contact with numerous bacterial stimuli also produce IL-7. Here we show that secretion of IL-7 by IECs is significantly suppressed upon stimulation by various bacterial components, including flagellin. Analysis of the intracellular mechanism by which flagellin regulates IL-7 production revealed that flagellin down-regulates expression of the two major transcripts encoding IL-7. Surprisingly, such function of flagellin was independent from the known transcriptional regulation of the IL-7 gene, as no significant change was observed in the transcriptional activity regulated by the previously identified promoter region. As the stability of IL-7 mRNA also remained unchanged upon flagellin stimulation, results suggested the possible involvement of a yet unknown transcriptional regulation of the IL-7 gene. These results describe a novel regulation of IL-7 production by bacterial stimuli, presumably mediated via Toll-like receptors. The present system might contribute to regulate the local lymphocyte pool, in response to the gut luminal or sub-mucosal bacterial abundance.

出版ステータスPublished - 2008 10月

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 生化学
  • 血液学
  • 分子生物学


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