TY - JOUR
T1 - FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan
AU - Kubota, Hiroaki
AU - Uwamino, Yoshifumi
AU - Matsui, Mari
AU - Sekizuka, Tsuyoshi
AU - Suzuki, Yasunori
AU - Okuno, Rumi
AU - Uchitani, Yumi
AU - Ariyoshi, Tsukasa
AU - Aoki, Wataru
AU - Suzuki, Satowa
AU - Kuroda, Makoto
AU - Shinkai, Takayuki
AU - Yokoyama, Keiko
AU - Sadamasu, Kenji
AU - Funakoshi, Takeru
AU - Murata, Mitsuru
AU - Hasegawa, Naoki
AU - Iwata, Satoshi
N1 - Funding Information:
We thank Miho Nishio and Norimi Kishi for technical assistance with the NGS experiments and Ken-ichi Lee and Masatomo Morita for technical advice at the preliminary stage of this study at the National Institute of Infectious Diseases, Japan. This work was supported by the Research Program on Emerging and Reemerging Infectious Diseases from the Japan Agency for Medical Research and Development, AMED (grant numbers: 17fk0108121j and 18fk0108048j), and by a grant for Research on Emerging and Re-emerging Infectious Diseases and Immunization from the Japanese Ministry of Health, Labour and Welfare (grant number: H28-Shinko Gyosei-Ippan-006).
Publisher Copyright:
© The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the ‘Big Five’ carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase. Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced. Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated. Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.
AB - Objectives: A carbapenem-resistant Enterobacter cloacae complex isolated in Tokyo, Japan, produced a carbapenemase that was detected by a Carba NP test and a modified carbapenem inactivation method, but none of the ‘Big Five’ carbapenemase genes was detected by PCR. This study aimed to identify the carbapenemase. Methods: Carbapenemase genes were screened by WGS. Next, we generated a recombinant plasmid in which the carbapenemase gene was inserted. We also extracted the carbapenemase gene-carrying plasmid from the E. cloacae complex. The effects of both plasmids on the antibiotic susceptibility of Escherichia coli were then tested. The carbapenemase gene-carrying plasmid in the E. cloacae complex was completely sequenced. Results: A novel carbapenemase gene, blaFRI-4, encoded an amino acid sequence that was 93.2% identical to French imipenemase (FRI-1). E. coli transformed with blaFRI-4 showed reduced carbapenem susceptibility. A complete sequence of the blaFRI-4-carrying 98 508 bp IncFII/IncR plasmid (pTMTA61661) showed that blaFRI-4 and the surrounding region (18.7 kb) were duplicated. Conclusions: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.
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U2 - 10.1093/jac/dky291
DO - 10.1093/jac/dky291
M3 - Article
C2 - 30060114
AN - SCOPUS:85055207804
SN - 0305-7453
VL - 73
SP - 2969
EP - 2972
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 11
ER -
