TY - JOUR
T1 - Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing
AU - Ishikawa, Tomohiro
AU - Isobe, Keisuke
AU - Inazawa, Kenta
AU - Michikawa, Takayuki
AU - Namiki, Kana
AU - Miyawaki, Atsushi
AU - Kannari, Fumihiko
AU - Midorikawa, Katsumi
N1 - Funding Information:
We are truly grateful to the Advanced Manufacturing Support Team at RIKEN Center for Advanced Photonics for manufacturing the transmission echelle grating. This work was supported by JST CREST (Grant Nos. JPMJCR1851 and JPMJCR1921), MEXT/JSPS KAKENHI (Grant No. JP18H04750 “Resonance Bio”), and the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering grant program. This work was also supported by the RIKEN Junior Research Associate Program.
Publisher Copyright:
© 2022 The Author(s). Published on behalf of The Japan Society of Applied Physics by IOP Publishing Ltd.
PY - 2022/4
Y1 - 2022/4
N2 - Multi-photon holographic patterned illumination using temporal focusing (TF) pulses provides 10 μm multi-spots with an axial confinement of 5 μm, enabling parallel photo-stimulation of multi-target cells in three dimensions. However, interference fringes and speckles degrade the uniformity of the spots, causing spatio-temporal fluctuations in photoactivation. Here, we demonstrate fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed (TM) TF pulses even when the multi-spots overlap spatially. The uniform patterned illumination enabled the expansion of the field-of-view of TM-TF microscopy by more than fivefold and enhanced the axial resolution to 1.3 μm.
AB - Multi-photon holographic patterned illumination using temporal focusing (TF) pulses provides 10 μm multi-spots with an axial confinement of 5 μm, enabling parallel photo-stimulation of multi-target cells in three dimensions. However, interference fringes and speckles degrade the uniformity of the spots, causing spatio-temporal fluctuations in photoactivation. Here, we demonstrate fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed (TM) TF pulses even when the multi-spots overlap spatially. The uniform patterned illumination enabled the expansion of the field-of-view of TM-TF microscopy by more than fivefold and enhanced the axial resolution to 1.3 μm.
KW - holographic patterned illumination
KW - multiphoton microscopy
KW - temporal focusing
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U2 - 10.35848/1882-0786/ac5dec
DO - 10.35848/1882-0786/ac5dec
M3 - Article
AN - SCOPUS:85128346336
SN - 1882-0778
VL - 15
JO - Applied Physics Express
JF - Applied Physics Express
IS - 4
M1 - 042005
ER -