@article{3c6b0d2c9d96460a9e34e09321a111f4,
title = "Functional glycosylation of human podoplanin: Glycan structure of platelet aggregation-inducing factor",
abstract = "Podoplanin (Aggrus) is a mucin-type sialoglycoprotein that plays a key role in tumor cell-induced platelet aggregation. Podoplanin possesses a platelet aggregation-stimulating (PLAG) domain, and Thr52 in the PLAG domain of human podoplanin is important for its activity. Endogenous or recombinant human podoplanin were purified, and total glycosylation profiles were surveyed by lectin microarray. Analyses of glycopeptides produced by Edman degradation and mass spectrometry revealed that the disialyl-corel (NeuAcα2-3Galβl-3(NeuAcα2-6)GalNAcαl-O-Thr) structure was primarily attached to a glycosylation site at residue Thr52. Sialic acid-deficient podoplanin recovered its activity after additional sialylation. These results indicated that the sialylated Corel at Thr52 is critical for podoplanin-induced platelet aggregation.",
keywords = "Disialyl-T, Disialyl-corel, Platelet aggregation, Podoplanin",
author = "Kaneko, {Mika Kato} and Yukinari Kato and Akihiko Kameyama and Hiromi Ito and Atsushi Kuno and Jun Hirabayashi and Tomomi Kubota and Koh Amano and Yasunori Chiba and Yasushi Hasegawa and Isoji Sasagawa and Kazuhiko Mishima and Hisashi Narimatsu",
note = "Funding Information: We thank Ms. Kahori Tachibana, Ms. Sanae Furukawa, Ms. Kozue Hagiwara, Ms. Yuki Tsunoda, and Mr. Noboru Uchiyama for their technical assistance. We are very grateful to Drs. Webster K Cavenee, Naoya Fujita, and Takashi Tsuruo for their generous donation of materials. This work was performed as a part of the Medical Glycomics Project supported by NEDO (New Energy and Industrial Technology Development Organization). This study was supported in part by a grant for Research Fellowships from the Japanese Society for the Promotion of Science (Y. Kato), by Kanae Foundation for Life and Socio-medical Science (Y. Kato), and by the Osaka Cancer Research Foundation (Y. Kato).",
year = "2007",
month = jan,
day = "23",
doi = "10.1016/j.febslet.2006.12.044",
language = "English",
volume = "581",
pages = "331--336",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",
}