Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes

Yuichi Uchino, Ashley M. Woodward, Jérôme Mauris, Kristoffer Peterson, Priya Verma, Ulf J. Nilsson, Jaya Rajaiya, Pablo Argüeso

研究成果: Article査読

22 被引用数 (Scopus)

抄録

Interleukin-1β (IL-1β) is a potent mediator of innate immunity commonly up-regulated in a broad spectrum of inflammatory diseases. When bound to its cell surface receptor, IL-1β initiates a signalling cascade that cooperatively induces the expression of canonical IL-1 target genes such as IL-8 and IL-6. Here, we present galectin-3 as a novel regulator of IL-1β responses in corneal keratinocytes. Using the SNAP-tag system and digitonin semi-permeabilization, we show that recombinant exogenous galectin-3 binds to the plasma membrane of keratinocytes and is internalized into cytoplasmic compartments. We find that exogenous galectin-3, but not a dominant negative inhibitor of galectin-3 polymerization lacking the N-terminal domain, exacerbates the response to IL-1β by stimulating the secretion of inflammatory cytokines. The activity of galectin-3 could be reduced by a novel d-galactopyranoside derivative targeting the conserved galactoside-binding site of galectins and did not involve interaction with IL-1 receptor 1 or the induction of endogenous IL-1β. Consistent with these observations, we demonstrate that small interfering RNA-mediated suppression of endogenous galectin-3 expression is sufficient to impair the IL-1β-induced secretion of IL-8 and IL-6 in a p38 mitogen-activated protein kinase-independent manner. Collectively, our findings provide a novel role for galectin-3 as an amplifier of IL-1β responses during epithelial inflammation through an as yet unidentified mechanism.

本文言語English
ページ(範囲)490-499
ページ数10
ジャーナルImmunology
154
3
DOI
出版ステータスPublished - 2018 7月

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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