Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa; Makoto Ogura; Shunichi Sato; Hitoshi Wakisaka; Hiroshi Ashida; Maki Uenoyama; Yoshinori Masaki; Minoru Obara

doi:10.1364/OL.29.001227

Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, Minoru Obara

電気情報工学科

研究成果: Article › 査読

67 被引用数 (Scopus)

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Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

本文言語	English
ページ（範囲）	1227-1229
ページ数	3
ジャーナル	Optics Letters
巻	29
号	11
DOI	https://doi.org/10.1364/OL.29.001227
出版ステータス	Published - 2004 6月 1

ASJC Scopus subject areas

原子分子物理学および光学

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10.1364/OL.29.001227

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AU - Sato, Shunichi

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AU - Ashida, Hiroshi

AU - Uenoyama, Maki

AU - Masaki, Yoshinori

AU - Obara, Minoru

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Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

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