@article{6296056c28be4b578f7cfbad21aaa5bd,
title = "Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors",
abstract = "Nakatake et al. establish 714 transgene-inducible human embryonic stem cell (hESC) lines and capture global transcriptome changes perturbed by the overexpression of these transgenes—mostly transcription factors (TFs). The analyses help in classifying TFs and identify sets of TFs that direct the differentiation of hESCs into distinct cell types.",
keywords = "cell differentiation, conditional induction, human embryonic stem cells, transcription factors, transcriptome",
author = "Yuhki Nakatake and Ko, {Shigeru B.H.} and Sharov, {Alexei A.} and Shunichi Wakabayashi and Miyako Murakami and Miki Sakota and Nana Chikazawa and Chiaki Ookura and Saeko Sato and Noriko Ito and Madoka Ishikawa-Hirayama and Mak, {Siu Shan} and Jakt, {Lars Martin} and Tomoo Ueno and Ken Hiratsuka and Misako Matsushita and Goparaju, {Sravan Kumar} and Tomohiko Akiyama and Ishiguro, {Kei ichiro} and Mayumi Oda and Norio Gouda and Akihiro Umezawa and Hidenori Akutsu and Kunihiro Nishimura and Ryo Matoba and Osamu Ohara and Ko, {Minoru S.H.}",
note = "Funding Information: We thank all laboratory members for discussion and helpful suggestions; Motohiko Tanino, Yukari Ikeda, Ken Nonaka, and Hiroshi Iijima (DNA Chip) for their technical help in RNA-seq and microarray data analysis; Masakazu Machida (NrichD) for help with the SEES3 hESC line; and Utsumi Noriko (Kazusa DNA Research Institute) for handling human ORFs. This work was mainly supported by the CREST program from the Japan Science and Technology Agency (JST, grant number JPMJCR12W4 ). This work was supported in part by the Keio University Medical Science Fund – The Mitsunada Sakaguchi Laboratory and the Collaborative Research Resources, School of Medicine, Keio University and Japan Agency for Medical Research and Development (AMED, grant number 17bm0404012h0005 ). Funding Information: We thank all laboratory members for discussion and helpful suggestions; Motohiko Tanino, Yukari Ikeda, Ken Nonaka, and Hiroshi Iijima (DNA Chip) for their technical help in RNA-seq and microarray data analysis; Masakazu Machida (NrichD) for help with the SEES3 hESC line; and Utsumi Noriko (Kazusa DNA Research Institute) for handling human ORFs. This work was mainly supported by the CREST program from the Japan Science and Technology Agency (JST, grant number JPMJCR12W4). This work was supported in part by the Keio University Medical Science Fund ? The Mitsunada Sakaguchi Laboratory and the Collaborative Research Resources, School of Medicine, Keio University and Japan Agency for Medical Research and Development (AMED, grant number 17bm0404012h0005). Y.N. M. Murakami, M.S. N.C. C.O. S.S. N.I. M.I.H. S.S.M. L.M.J. T.U. K.H. M. Matsushita, S.K.G. T.A. K.-i.I. M.O. R.M. and O.O. carried out the experiments; Y.N. A.A.S. S.W. L.M.J. K.N. N.G. and M.S.H.K. carried out the data analysis; M.S.H.K. conceived the project; A.U. and H.A. provided a human ESC line (SEES3); O.O. provided most of the transgenes; S.B.H.K. H.A. K.N. R.M. O.O. and M.S.H.K. supervised the project; and Y.N. and M.S.H.K. wrote the manuscript with inputs from all authors. M.S.H.K. is a founder of Elixirgen Scientific, which receives a patent license from Keio University and commercializes transcription factor-based human pluripotent stem cell differentiation reagents. A.A.S. and N.C. are currently employed by Elixirgen Scientific. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = may,
day = "19",
doi = "10.1016/j.celrep.2020.107655",
language = "English",
volume = "31",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "7",
}