TY - JOUR
T1 - Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
AU - Yoshimatsu, Sho
AU - Murakami, Rei
AU - Sato, Tsukika
AU - Saeki, Tsubasa
AU - Yamamoto, Masafumi
AU - Sasaki, Erika
AU - Noce, Toshiaki
AU - Okano, Hideyuki
N1 - Funding Information:
We would like to express our thanks to Yuka Hiroi (Keio Univeristy) for assistance with this study. We also thank all the laboratory members of H.O. for their encouragement and generous support. Data presented herein were obtained in the “Construction of System for Spread of Primate Model Animals” project, performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP20dm0207001 to H.O.); and Scientific Research in Innovative Areas, a MEXT Grant-in-Aid project FY2014-2018 “Brain Protein Aging and Dementia Control” (Grant ID: 26117007 to H.O.). This work was also supported by Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.) and JSPS KAKENHI Grant Number 19J12871 and 20K22660 (to S.Y).
Publisher Copyright:
© 2021 The Authors
PY - 2021/5
Y1 - 2021/5
N2 - POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).
AB - POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).
UR - http://www.scopus.com/inward/record.url?scp=85104952207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85104952207&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2021.102308
DO - 10.1016/j.scr.2021.102308
M3 - Article
C2 - 33799281
AN - SCOPUS:85104952207
SN - 1873-5061
VL - 53
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 102308
ER -